pf4 concentration  (Boster Bio)

Journal: Frontiers in Aging Neuroscience

Article Title: Evaluation of the efficacy of Tai Chi on the cognitive function of patients with mild cognitive dysfunction and research on its mechanism

doi: 10.3389/fnagi.2025.1435996

Figure Lengend Snippet: Comparison of general and baseline data among the three groups of patients ( N = 54).

Article Snippet: Our study is the first exploratory trial that utilizes PF4 as a serum concentration indicator for detecting MCI patients after exercise (Tai Chi or walking exercise). however, unfortunately, the concentration of serum PF4 levels in this study did not show an increase in the concentration of serum PF4 levels due to the intervention of the exercise, but instead, there was a trend of a slight decrease in the levels of PF4 with the completion of the intervention; suggesting that the effect of Tai Chi or walking exercises on the serum PF4 concentrations in MCI patients had no effect because, with age, PF4 in human and non-human primate plasma gradually decreases ( ).

Techniques: Comparison

Journal: Frontiers in Aging Neuroscience

Article Title: Evaluation of the efficacy of Tai Chi on the cognitive function of patients with mild cognitive dysfunction and research on its mechanism

doi: 10.3389/fnagi.2025.1435996

Figure Lengend Snippet: Analysis of serum PF4 results in three groups of patients ( n = 52).

Article Snippet: Our study is the first exploratory trial that utilizes PF4 as a serum concentration indicator for detecting MCI patients after exercise (Tai Chi or walking exercise). however, unfortunately, the concentration of serum PF4 levels in this study did not show an increase in the concentration of serum PF4 levels due to the intervention of the exercise, but instead, there was a trend of a slight decrease in the levels of PF4 with the completion of the intervention; suggesting that the effect of Tai Chi or walking exercises on the serum PF4 concentrations in MCI patients had no effect because, with age, PF4 in human and non-human primate plasma gradually decreases ( ).

Techniques:

Journal: Frontiers in Aging Neuroscience

Article Title: Evaluation of the efficacy of Tai Chi on the cognitive function of patients with mild cognitive dysfunction and research on its mechanism

doi: 10.3389/fnagi.2025.1435996

Figure Lengend Snippet: Results of serum PF4 in three groups of patients ( n = 52).

Article Snippet: Our study is the first exploratory trial that utilizes PF4 as a serum concentration indicator for detecting MCI patients after exercise (Tai Chi or walking exercise). however, unfortunately, the concentration of serum PF4 levels in this study did not show an increase in the concentration of serum PF4 levels due to the intervention of the exercise, but instead, there was a trend of a slight decrease in the levels of PF4 with the completion of the intervention; suggesting that the effect of Tai Chi or walking exercises on the serum PF4 concentrations in MCI patients had no effect because, with age, PF4 in human and non-human primate plasma gradually decreases ( ).

Techniques:

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Carbon dioxide alleviates platelet storage lesions via stimulating fatty acid metabolism and reducing platelet glucose consumption

doi: 10.1016/j.rpth.2025.102681

Figure Lengend Snippet: Carbon dioxide (CO 2 ) inhibited platelet activation and apoptosis during storage. (A) The percentage of CD62P-positive platelets was determined by flow cytometry. (B) Platelet factor 4 (PF4) concentration was determined by enzyme-linked immunosorbent assay. (C) The percentage of annexin V-positive platelets was determined by flow cytometry. (D) The mean fluorescence intensity (MFI) of CD42b was determined by flow cytometry ( n = 6). n.s., not significant, ∗ P < .05, ∗∗ P < .01, ∗∗∗ P < .001, ∗∗∗∗ P < .0001.

Article Snippet: To determine PF4 concentration, 200 μL of supernatant from stored APCs (300 μL, 1 × 10 9 /mL) was processed following the PF4 ELISA Kit instructions (Boster).

Techniques: Activation Assay, Flow Cytometry, Concentration Assay, Enzyme-linked Immunosorbent Assay, Fluorescence

Journal: Molecular medicine (Cambridge, Mass.)

Article Title: CXCL4 deficiency limits M4 macrophage infiltration and attenuates hyperoxia-induced lung injury.

doi: 10.1186/s10020-024-01043-y

Figure Lengend Snippet: Fig. 1 M4 macrophages are activated and CXCL4 is upregulated in BPD. (A) Representative images of H&E staining (arrow represented the pathological structural change in the lung tissues of mice) and IF for F4/80 (a macrophage marker), S100A8, MMP7, and DAPI in lung tissues from NOX (21% O2) and HYX (95% O2) groups (n = 6). (B) Flow cytometric analysis showing the percentage of F4/80+S100A8+MMP7+ cells in NOX and HYX groups (n = 6). (C-D) qRT-PCR analysis and ELISA quantification of CXCL4 expression levels (n = 6). (E) IF staining for F4/80 and CXCL4 in lung tissues (n = 6). (F-G) Western blot analysis of CXCL4, TNF-α, IL-6, MMP7, S100A8, and GAPDH in lung tissues from NOX and HYX groups (n = 6)

Article Snippet: CXCL4 concentrations were quantified with a mouse CXCL4/PF-4 ELISA kit (MCX400, R&D Systems, USA).

Techniques: Staining, Marker, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot

Journal: Molecular medicine (Cambridge, Mass.)

Article Title: CXCL4 deficiency limits M4 macrophage infiltration and attenuates hyperoxia-induced lung injury.

doi: 10.1186/s10020-024-01043-y

Figure Lengend Snippet: Fig. 2 Loss of CXCL 4 alleviates hyperoxia-induced alveolar epithelial type 2 cells (AT2) injury and suppresses S1P metabolism. (A) H&E staining of lung tissues from WT and CXCL4 KO mice exposed to NOX (21% O2) and HYX (95% O2) conditions for 7 days (n = 6). (B) Quantification of the average surface area of a single alveolus (µm²) (n = 6). (C) Radial Alveolar Count (RAC) analysis (n = 6). (D) Representative images of TUNEL staining (green), SFTPC (red), and DAPI (blue) in lung sections, indicating apoptosis and AT2 cell distribution (n = 6). (E) Quantification of TUNEL-positive cells (n = 6). (F) Quantification of SFTPC + cells per field of view (n = 6). (G-H) Western blot analysis of SPHK1, SPHK2, SPT2, and S1PL, genes associated with sphingosine-1-phosphate metabolism (n = 6)

Article Snippet: CXCL4 concentrations were quantified with a mouse CXCL4/PF-4 ELISA kit (MCX400, R&D Systems, USA).

Techniques: Staining, TUNEL Assay, Western Blot

Journal: Molecular medicine (Cambridge, Mass.)

Article Title: CXCL4 deficiency limits M4 macrophage infiltration and attenuates hyperoxia-induced lung injury.

doi: 10.1186/s10020-024-01043-y

Figure Lengend Snippet: Fig. 3 Deletion of CXCL4 weakens fibrotic lung remodeling. (A) Representative images of lung tissue sections from WT and CXCL4 KO mice under NOX (21% O2) and HYX (95% O2) conditions (n = 6). (B) Measurement of septal thickness (µm) in the NOX_WT, NOX_CXCL4 KO, HYX_WT, and HYX_CXCL4 KO groups (n = 6). (C) Analysis of elastic fibers relative to lung tissue (n = 6). (D) Sirius Red staining showing changes in collagen content (n = 6). (E) Quan tification of collagen amount relative to lung tissue (n = 6). (F) Western blot analysis of phosphorylated SMAD2 (n = 6). (G) Relative expression levels of p-SMAD2/SMAD2 protein ratio (n = 6)

Article Snippet: CXCL4 concentrations were quantified with a mouse CXCL4/PF-4 ELISA kit (MCX400, R&D Systems, USA).

Techniques: Staining, Western Blot, Expressing

Journal: Molecular medicine (Cambridge, Mass.)

Article Title: CXCL4 deficiency limits M4 macrophage infiltration and attenuates hyperoxia-induced lung injury.

doi: 10.1186/s10020-024-01043-y

Figure Lengend Snippet: Fig. 4 Deletion of CXCL4 prevents the progression of M4 macrophages in the lung. (A) IF staining for F4/80, S100A8, and DAPI in lung tissues from WT and CXCL4 KO mice under NOX (21% O2) and HYX (95% O2) conditions (n = 6). (B) IF staining for F4/80, MMP7, and DAPI (n = 6). (C) Flow cytometry analysis quantifying F4/80 + S100A8 + MMP7 + cells (%) (n = 6). (D-E) Western blot analysis of CXCL4, TNF-α, IL-6, MMP7, S100A8, and GAPDH (n = 6)

Article Snippet: CXCL4 concentrations were quantified with a mouse CXCL4/PF-4 ELISA kit (MCX400, R&D Systems, USA).

Techniques: Staining, Flow Cytometry, Western Blot

Journal: Molecular medicine (Cambridge, Mass.)

Article Title: CXCL4 deficiency limits M4 macrophage infiltration and attenuates hyperoxia-induced lung injury.

doi: 10.1186/s10020-024-01043-y

Figure Lengend Snippet: Fig. 5 CXCL4 induces M4 macrophages in vitro and drives macrophage migration via CCR 1. (A-B) Expression levels of S100A8 and MMP7 in macro phages treated with M-CSF (M0) and CXCL4 (M4) (n = 3). (C-D) Dose-dependent expression of MMP7 and S100A8 in response to varying concentrations of CXCL4 (4, 2, 1, 0.5 µM) (n = 3). (E) IF staining of S100A8 and DAPI (n = 3). (F) IF staining of MMP7 and DAPI (n = 3). (G-H) ELISA results for MMP7 and S100A8 (n = 3). (I-J) Macrophage migration assay comparing control, CXCL4, CCR1 inhibitor (CCR1 Inhi), and CXCL4 + CCR1 Inhi groups. Arrows represented the type of migrated cells (n = 3)

Article Snippet: CXCL4 concentrations were quantified with a mouse CXCL4/PF-4 ELISA kit (MCX400, R&D Systems, USA).

Techniques: In Vitro, Migration, Expressing, Staining, Enzyme-linked Immunosorbent Assay, Control

Journal: Molecular medicine (Cambridge, Mass.)

Article Title: CXCL4 deficiency limits M4 macrophage infiltration and attenuates hyperoxia-induced lung injury.

doi: 10.1186/s10020-024-01043-y

Figure Lengend Snippet: Fig. 6 CXCL4 deficiency promotes lung matrix remodeling during regeneration. (A) H&E staining of lung tissues from WT and CXCL4 KO mice exposed to HYX followed by a recovery period under NOX conditions (n = 6). (B) Measurement of the average surface area of a single alveolus (µm²) (n = 6). (C) Radial Alveolar Count (RAC) analysis (n = 6). (D) Measurement of septal thickness (µm) (n = 6). (E-F) Quantification of collagen amount relative to lung tissue (n = 6)

Article Snippet: CXCL4 concentrations were quantified with a mouse CXCL4/PF-4 ELISA kit (MCX400, R&D Systems, USA).

Techniques: Staining